解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The concentration of myelin basic protein (MBP) mRNA in primary cultures of cells dissociated from embryonic mouse cerebra and grown in the presence of varying amounts of thyroid hormone was measured using a 32P-labeled cDNA probe and a dot-blot procedure. The cDNA probe contained 1.85 kilobases of the gene for MBP. T...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170304
更新日期:1987-01-01 00:00:00
abstract::Growth hormone (GH) appears to play a major role in a reciprocal axis that has been postulated between the thymus and pituitary glands. Our previous studies showed that thymic structure, as well as T-cell proliferation and IL-2 synthesis, could be restored in aged female Wistar-Furth rats by the implantation of GH3 pi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180118
更新日期:1987-01-01 00:00:00
abstract::The effects of ischemia (removal of oxygen and glucose for 4 h) and anoxia (removal of oxygen alone) on astrocytes were studied in dissociated cultures of E14 spinal cord containing both neurons and astrocytes. In addition, a group of cultures was treated with a low Na+, low Ca2+, and high K+ medium during the 4-h isc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170212
更新日期:1987-01-01 00:00:00
abstract::Using [3H]-dopamine (DA) as the ligand of choice, we have shown that rat lymphocytes possess DA binding sites. The binding of [3H]-DA was specific, saturable, and of high affinity. The specific binding in membranes obtained from Con A-activated lymphoblasts was greater than the binding in respective unactivated lympho...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180112
更新日期:1987-01-01 00:00:00
abstract::The rationale for designing and synthesizing 24 different peptide analogs and subpeptides of residues 69-84 of guinea pig myelin basic protein is presented. The encephalitogenic potential of 19 of these peptides in Lewis rats is also given. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180130
更新日期:1987-01-01 00:00:00
abstract::Sympathetic noradrenergic nerve fibers, stained with antiserum for tyrosine hydroxylase (TH), richly innervate the splenic white pulp. These fibers distribute with the vascular and trabecular systems, and associate mainly with the central artery and its branches, the periarteriolar lymphatic sheath (PALS), the margina...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180107
更新日期:1987-01-01 00:00:00
abstract::Fourteen-day gestation fetal cerebral cortex homografts were transplanted into the thoracic (T6) spinal cord between the left dorsal column and dorsal horn of adult host rats. The transplants were soaked in 2.0 micrograms/ml of the lectin Phaseolus vulgaris leucoagglutinin (PHAL) prior to implantation. Transplanted ho...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170410
更新日期:1987-01-01 00:00:00
abstract::Contrary to widely held beliefs, the gene encoding proteolipid protein (PLP), the major structural protein of central nervous system myelin, is expressed in Schwann cells and their tumors. Proteolipid mRNA was identified in human acoustic neuromas and in rat and rabbit sciatic nerves using a human PLP cDNA as a probe....
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180402
更新日期:1987-01-01 00:00:00
abstract::Substance P (SP) acts as an immunoregulator by binding to specific functional cell surface receptors on a subpopulation of human T-helper lymphocytes. Receptors with similar properties have also been characterized on the human IM-9 B-lymphoblast cell line. Four distinct proteins of molecular weight (MW) 33,000, 58,000...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180127
更新日期:1987-01-01 00:00:00
abstract::The present study examined trophic activities in normal and injured brain which affect the survival and growth of central neurons in culture. Adult rats received bilateral lesions through the angular bundle, severing projections between the entorhinal cortex and the hippocampus. Ten days later, extracts were prepared ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180203
更新日期:1987-01-01 00:00:00
abstract::The effects of cations on the spontaneous and potassium-stimulated taurine release from mouse cerebral cortex slices were assessed with an emphasis on the as yet unestablished calcium dependence of the stimulated release. Spontaneous and stimulated GABA release was analyzed for comparison. A depolarizing concentration...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170316
更新日期:1987-01-01 00:00:00
abstract::Transferrin is the second most abundant plasma protein and functions to transport iron. It is an essential constituent in culture media for virtually all cells. In a recent study, we reported that transferrin (Tf) is specifically located in oligodendrocytes in the rat nervous system. This investigation examines immuno...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170108
更新日期:1987-01-01 00:00:00
abstract::Intact synaptosomes prepared from 28-day-old rat brains were incubated with CMP-N-acetyl-(14C) neuraminic acid in Krebs-Henseleit buffer in an atmosphere of 95% O2: 5% CO2, at 37 degrees C. The activity of CMP-NANA:ganglioside sialosyltransferase using endogenous acceptors was 0.84 pmoles NANA transferred/mg synaptoso...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180312
更新日期:1987-01-01 00:00:00
abstract::Larval acetylcholinesterase (acetylcholine acetylhydrolase) EC 3.1.3.7 of the trematode Schistosoma mansoni was characterized and purified by affinity chromatography. The enzyme was solubilized from sonicated cercarial tissue and showed a Km value of 1.83 mM and a Vmax value of 102 U/mg protein. It was characterized a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150413
更新日期:1986-01-01 00:00:00
abstract::The postmortem stability of brain RNA was measured in rat and human samples for up to 48 hr. Whole rat brains, cooled at a rate approximating that of human brains awaiting autopsy, were collected at intervals from 0 to 48 hr after death and frozen. These samples were compared with freshly obtained, unfrozen rat brains...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490160123
更新日期:1986-01-01 00:00:00
abstract::Previously we reported results of an incubation experiment with neurofilaments that supported the existence of a mu-type of Ca2+-activated neutral protease in the rat peripheral nerve; it was active with microM order Ca2+ (mu-CANP). This time, we partially purified the mu-CANP from a crude CANP fraction of rat periphe...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150206
更新日期:1986-01-01 00:00:00
abstract::In order to quantify the changes that occur in the cholinergic central nervous system with aging, we have compared acetylcholine (Ach) formation in brain cortex slice preparations from 2-year-old aged CBF-1 mouse brains and compared the findings with those in 2-4-month-old young adult mouse brain slices. Incorporation...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150209
更新日期:1986-01-01 00:00:00
abstract::The influence of a concentrated extract of soluble substances from the sciatic nerve upon the acetylcholine receptor (AChR) content in the soleus muscle of adult rats was examined by in vivo infusions. Internal and membrane-inserted AChR were quantitated by the specific binding of 125I-alpha-bungarotoxin (a-BuTX). Int...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490160307
更新日期:1986-01-01 00:00:00
abstract::The properties of neuronal plasma membranes are highly specialized, since they include the receptors by which many hormones, neurotransmitters, drugs, and toxins initiate their actions, as well as the components necessary for receiving synaptic input from a variety of sources and for generating the appropriate pattern...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150412
更新日期:1986-01-01 00:00:00
abstract::In situ cDNA:mRNA hybridization is a technique that has been developed for the visualization of cDNA:mRNA hybrids in individual cells. To use this technique to answer questions of regulation in heterogeneous populations of cells in the brain, it must be combined with other procedures allowing for the identification of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490160109
更新日期:1986-01-01 00:00:00
abstract::Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5%, similar binding of non-antibody-producing cells is 4-7%, compared to the binding of the hybridoma...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150312
更新日期:1986-01-01 00:00:00
abstract::Glial fibrillary acidic protein (GFAP) is the major constituent of glial filaments and is restricted within the CNS to astrocytes. As with other classes of intermediate filament proteins, the regulation of GFAP expression is poorly understood. Utilizing highly purified cultures of astrocytes and a chemically defined (...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490140202
更新日期:1985-01-01 00:00:00
abstract::Glycolysis was blocked in the hippocampus of the otherwise unaffected rabbit by perfusion of a previously implanted thin dialysis tube with a medium containing 0.1 mM iodoacetate. The perfusate from the dialysis tube was analyzed for free amino acids with liquid chromatographic separation of ortophthaldialdehyde deriv...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130403
更新日期:1985-01-01 00:00:00
abstract::Bovine cerebrum, including the corpus callosum, and a human oligodendroglioma were investigated by an immunohistochemical technique to determine the distribution of 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP). Also, three human oligodendrogliomas (ODG) were characterized by an immunoblot procedure to identify a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130309
更新日期:1985-01-01 00:00:00
abstract::This essay discusses the directional movements of metazoan tissue cells generally, with special emphasis on neurons, in an attempt to show that the directional movements of all share fundamental similarities. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.490130102
更新日期:1985-01-01 00:00:00
abstract::The rate and direction of neurite growth have been shown in a number of studies to be determined by the distribution of adhesive sites on the growth cone. Recent evidence showing that the application of extrinsic electric fields can redistribute membrane molecules and alter both the rate and direction of neurite growt...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130118
更新日期:1985-01-01 00:00:00
abstract::Extracts of bovine heart, rat heart, and rat skeletal muscle were chromatographically separated and the fractions tested for their ability to maintain the survival of dissociated sympathetic, parasympathetic, and sensory neurones. Bovine heart contained at least five, rat heart at least six, and rat skeletal muscle at...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130303
更新日期:1985-01-01 00:00:00
abstract::The phosphorylative neuromodulation of the regulatory subunit of protein kinase type II (R-II) in cytosolic fractions from denervated and sham-operated, contralateral soleus muscles of the rat was evaluated. The denervation-induced increase in the 32P-phosphorylation of R-II is not related to an increased dephosphoryl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490140208
更新日期:1985-01-01 00:00:00
abstract::The potassium-induced stimulation of oxygen consumption in brain slices has a threshold value of 15-20 mM potassium, and it reaches its maximum at 35-50 mM. Although this phenomenon now has been known for almost 50 years, its physiological role remains undetermined. One reason for this may be that the high concentrati...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490140108
更新日期:1985-01-01 00:00:00
abstract::The extension of filopodia from growth cones of regenerating neurites from rat superior cervical ganglion neurons in tissue culture was studied. Cultures were grown on a thin layer of fibrous collagen and maintained in a medium containing serum and nerve growth factor. Time-lapse cinematography and computer-assisted m...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130111
更新日期:1985-01-01 00:00:00
abstract::The activity levels of pyruvate dehydrogenase, enzymes of the citric acid cycle, aspartate and alanine aminotransferases, and NADP+-isocitrate dehydrogenase were determined in the cerebral cortex, cerebellum, brain stem, corpus striatum, hippocampus, and midbrain regions of normal rats and rats injected with acute and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490140407
更新日期:1985-01-01 00:00:00
abstract::Abdominal muscle electromyograms were monitored in response to graded mechanical loads added to either inspiration or expiration in anesthetized cats. The surgical preparation, the apparatus, and the levels of loads applied (resistive, tracheal occlusion, and continuous positive pressure) were matched with those emplo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130413
更新日期:1985-01-01 00:00:00
abstract::There are two types of calcium-activated neutral protease (CANP), m-CANP and mu-CANP, following the nomenclature of Suzuki et al to show that each requires mM and microM Ca2+, respectively, for its activation. We found mu-CANP activity in a crude CANP fraction extracted from the peripheral nerve, which degraded the ne...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130306
更新日期:1985-01-01 00:00:00
abstract::Photopic L-type responses were recorded from the soma and the axon terminal of horizontal cells of the catfish (Ictalurus punctatus) retina in eye-cup preparations. The responses were produced by a spot of light with 100-micron diameter (intensity, 10 microW/cm2), which was flashed or steadily illuminated and swept al...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110405
更新日期:1984-01-01 00:00:00
abstract::A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophilic composition. Hematosides, GM3 and GD3, each showing double bands on thin-layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490120208
更新日期:1984-01-01 00:00:00
abstract::Recent findings suggest that exogenous gangliosides improve recovery of a learned behavior (alternation in a T-maze) which is thought to be related to sprouting after lesions of the entorhinal cortex. In the present investigation, we studied an unlearned behavior (the open-field hyperactivity resulting from bilateral ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490120228
更新日期:1984-01-01 00:00:00
abstract::Nicotinates, pantothenates, riboflavin, vitamins B6 and B12' free (acetyl) and total (free and bound) choline, biopterin, thiamin, biotin, methylated and nonmethylated folates in frontal, temporal, precentral, postcentral, and occipital cortex, thalamus, cerebellum, pons, basal ganglia, and substantia nigra were estim...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110409
更新日期:1984-01-01 00:00:00
abstract::In the present study we report self-assembly of individual neurofilament (NF) triplet proteins (70K, 150K, and 200K daltons) isolated by anion exchange chromatography from bovine spinal cord. Formation of smooth 10-nm filaments by both NF 150K and NF 70K is shown. Optimal conditions for NK 150K filament formation were...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110204
更新日期:1984-01-01 00:00:00
abstract::The effects of exogenous GM1 and GD1a on S20Y murine neuroblastoma cells were assessed by monitoring morphology, tumorigenicity, mitotic index, and plating efficiency. S20Y cells were seeded at a density equivalent to 5 X 10(4) cells per 35-mm tissue culture dish; 38-42 hr after seeding (preconfluent stage) the cells ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490120106
更新日期:1984-01-01 00:00:00
abstract::We studied the effects of oxygen-derived free radicals on the ultrastructure of brain cortical slices and the release of fatty acids from phospholipids of crude synaptosomes. Xanthine oxidase, hypoxanthine, and ADP-Fe3+, a free-radical-generating system, caused swelling of cellular processes and mitochondria. The oxyg...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490120408
更新日期:1984-01-01 00:00:00